Overproduction and Characterization of UvrA Protein Encoded by Plasmid from Acetobacter estunensis GP2

Autoři

  • P. Grones Department of Molecular Biology, Comenius University, Bratislava
  • S. Mináriková-Vávrová Department of Molecular Biology, Comenius University, Bratislava
  • M. Babič Department of Molecular Biology, Comenius University, Bratislava
  • Z. Odnogová Department of Molecular Biology, Comenius University, Bratislava
  • J. Grones Department of Molecular Biology, Comenius University, Bratislava

Abstrakt

Plasmid pGP2 from Acetobacter estunensis GP2 encodes 1 296 bp uvrA gene for 432 aa reparation protein exinuclease A. Bioinformatic analysis of the protein confirmed 13 ?-helixes and 14 ?-structures, two domains of ABC transporters, one GTPase domain and a 21-amino-acid large trans-membrane domain. The protein has 9888 % identities with some bacterial genome-encoded proteins. uvrA gene amplified by PCR was cloned in pET28a- expression vector and purified by HPLC affinity chromato¬graphy. The purified protein (47.17 kDa) shows the ATPase activity ten times lower than the Rep34 protein (isoletectric point 6.8) from plasmid pGP2.

Publikováno

15.09.2012

Jak citovat

Grones, P., Mináriková-Vávrová, S., Babič, M., Odnogová, Z., & Grones, J. (2012). Overproduction and Characterization of UvrA Protein Encoded by Plasmid from Acetobacter estunensis GP2. Chemické Listy, 106(8). Získáno z http://ww.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/876

Číslo

Sekce

Články