A Model of Cerebral Vasospasms and Metabolomic Mapping

Authors

  • A. Hejčl Neurochirurgic Clinic, University of J. E. Purkyne, Krajska Zdravotni, a.s., Masaryk Hospital in Usti nad Labem, Ústí nad Labem, Czech Republic | The International Clinical Research Center of St. Anne's University Hospital, Brno, Czech Republic | Department of Neuroscience, Institute of Experimental Medicine, v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • K. Vondráková Department of Developmental Epileptology, Institute of Physilogy, v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic | Department of Physiology, Faculty of Science, Charles University in Prague, Prague, Czech Republic
  • P. Kelbich Department of Biochemistry, Krajska Zdravotni, a.s., Masaryk Hospital in Usti nad Labem, Ústí nad Labem, Czech Republic
  • M. Sameš Neurochirurgic Clinic, University of J. E. Purkyne, Krajska Zdravotni, a.s., Masaryk Hospital in Usti nad Labem, Ústí nad Labem, Czech Republic
  • G. Tsenov Department of Developmental Epileptology, Institute of Physilogy, v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic | Department of Neurosciences, Biomedicine and Movement Sciences, Anatomy and Histology Section, Faculty of Medicine, University of Verona, Verona, Italy
  • P. Kačer University of Chemistry and Technology, Prague, Czech Republic

Keywords:

cerebral vasospasm, microdialysis, endothelin-1, HPLC, MS

Abstract

Cerebral vasospasm (CV) is a one of the main factors leading to high morbidity and mortality in patients with a severe subarachnoid hemorrhage. Biomarkers of early diagnosis of the vasospasm development and progression are still poorly understood. Use of microdialysis (MD) techniques combined with modern practices in metabolomic analysis can significantly contribute to the understanding of these phenomena. The aim of this study was to design an animal model of the CV for understanding biochemical correlates of CVs, their development and progression. Intrahippocampal infusion of endothelin-1, a potent vasoconstrictor, in adult rats was used to mimic CV-condition. A range of biomarkers relevant to clinical practice was monitored using the MD and the HPLC-MS analysis. As a result, we developed a suitable model and a selective method of analysis to monitor neurochemical correlates that are highly relevant for future experimental and clinical research.

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Published

2017-01-15

How to Cite

Hejčl, A., Vondráková, K., Kelbich, P., Sameš, M., Tsenov, G., & Kačer, P. (2017). A Model of Cerebral Vasospasms and Metabolomic Mapping. Chemické Listy, 111(1), 56–61. Retrieved from http://ww.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/113

Issue

Vol. 111 No. 1 (2017)

Section

Articles