Preparation of Certified Methodology for Testing Transdermal Absorption of Chemicals In Vitro

Authors

  • Lenka Kotingová Department of Preventive Medicine, Medical Faculty in Hradec Králové, Charles University, Hradec Králové, Czech Republic https://orcid.org/0000-0001-7714-7308
  • Zora Nývltová VÚOS, Rybitví, Czech Republic
  • Zdeňka Rösslerová VÚOS, Rybitví, Czech Republic
  • Lucie Bíšková VÚOS, Rybitví, Czech Republic
  • Jana Volková VÚOS, Rybitví, Czech Republic
  • Aleš Fibír Division of Plastic Reconstructive Surgery and Burns Treatment, Department of Surgery, University Hospital Hradec Králové, Hradec Králové, Czech Republic https://orcid.org/0000-0001-5294-3519
  • Jaroslava Kořínková Institute of Environmental and Chemical Engineering, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic https://orcid.org/0000-0002-0600-0170
  • Lenka Moravcová Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic
  • Petra Plodíková VÚOS, Rybitví, Czech Republic

DOI:

https://doi.org/10.54779/chl20220751

Keywords:

skin absorption, in vitro, diffusion cells, human skin, pig skin, caffeine, benzoic acid, testosterone

Abstract

This publication presents the preparation of a certified methodology for in vitro transdermal absorption testing of chemicals and highlights the various pitfalls in their implementation. Vertical diffusion cells (Franz cells) were used to test the dermal absorption of caffeine, benzoic acid, and testosterone across a penetration membrane (porcine ear skin), while the receptor fluid samples were evaluated by HPLC. The designed methodology was certified in 2022 in the Good Laboratory Practice system and will be used at the VUOS Rybitví and at the Medical Faculty of the Charles University in Hradec Králové to assess the dermal absorption of various substances in the environment and occupational surroundings.

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Published

2022-12-15

How to Cite

Kotingová, L., Nývltová, Z., Rösslerová, Z., Bíšková, L., Volková, J., Fibír, A., Kořínková, J., Moravcová, L., & Plodíková, P. (2022). Preparation of Certified Methodology for Testing Transdermal Absorption of Chemicals In Vitro. Chemické Listy, 116(12), 751–758. https://doi.org/10.54779/chl20220751

Issue

Vol. 116 No. 12 (2022)

Section

Articles